![]() ![]() Place the blot onto a piece of clean filter paper to dry. We compared several alternative ECL solutions for Western blot detection of endogenous proteins in whole cell lysates using inexpensive, commercially available reagents. Wash the blot with wash buffer 35 times for 5 minutes each. A cost effective non-commercial ECL-solution for Western blot detections yielding strong signals and low background. Place the blot in diluted fluorescent dye-labeled secondary antibody solution and incubate for 1 hour with gentle agitation. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Prepare the substrate according to manufacturer’s instructions.Immobilon® ECL Ultra substrate is compatible with both PVDF andnitrocellulose blotting membranes, as well as commonly used buffers and blocking reagents. High signal intensity makes Amersham ECL Select suitable for the most demanding Western blotting applications, allowing for detection of minute protein quantities. The intense signal created permits sensitive detection at the mid-to-low femtogram range using x-ray film or digital imaging systems. Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection. solution at 0 ☌, followed by heating at 50 ☌. Immobilon® ECL Ultra Western HRP Substrate is an ultra-sensitive reagent with long signal duration for the detection of horseradish peroxidase (HRP)-labeled antibodies on western blots. Western blotting is the most extensively used technique for the identification and characterisation of proteins and their expression levels. GE Healthcare with its various Amersham ECL kits and Thermo Fisher Pierce SuperSignal West kits dominate the provision of this Western blotting reagent. Immobilon ECL Ultra Western HRP Substrate When performing Western blotting, use a sufficient amount of blocking buffer, washing buffer, and chemiluminescent substrate. ![]()
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